In vivo anti-tumor activity of murine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene

JIAN MIN YANG; MICHAEL S FRIEDMAN; MARIANNE T HUBEN; JENNIFER FULLER; QIAO LI; ALFRED E CHANG; JAMES J MULE; KEVIN T MCDONAGH

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In vivo anti-tumor activity of murine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene

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We have confirmed efficient anti-tumor activities of the peripheral lymphocytes transduced with a p185HER2-specific chimeric T-cell receptor gene both in murine and in human in our previous studies. To further test the feasibility of chimeric T-cell receptor in a bone marrow transplantation model, we first, made two murine tumor cell lines: MT901 and MCA-205, to express human p185HER2 by retroviral gene transduction. Murine bone marrow cells were retrovirally transduced to express the chimeric T-cell receptor and gene-modified bone marrow cells were transplanted into lethally irradiated mouse. Six months post transplantation, p185HER2-positive tumor cells:MT-901/HER2 or MCA-205/ HER2 was subcutaneously or intravenously injected to make mouse models simulating primary breast cancer or pulmonary metastasis. The in vivo anti-tumor effects were monitored by the size of the subcutaneous tumor or counting the tumor nodules in the lungs after India ink staining. The size of the subcutaneous tumor was significantly inhibited and the number of pulmonary nodules were significantly decreased in mouse recipients transplanted with chimeric T-cell receptor modified bone marrow cells compared with the control group. Our results suggest the efficient in vivo anti-tumor activities of chimeric T-cell receptor gene modified bone marrow cells.

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《中华微生物学和免疫学：英文版》 |2006年第2期|117-124|共8页
作者
JIAN MIN YANG; MICHAEL S FRIEDMAN; MARIANNE T HUBEN; JENNIFER FULLER; QIAO LI; ALFRED E CHANG; JAMES J MULE; KEVIN T MCDONAGH;
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作者单位

Department of Hematology;

Changhai Hospital;

the Second Military Medical University;

Shanghai;

P. R. China;

Department of Internal Medicine;

University of Michigan Medical School;

Ann Arbor;

Michigan;

USA;

Department of Surgery;

University of Michigan Medical School;

Ann Arbor;

Michigan;

USA;

Division of Hematology;

Oncology;

and Blood and Marrow Transplantation;

Department of Internal Medicine;

University of Kentucky College of Medicine;

Lexington;

Kentucky;

USA;

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原文格式 PDF
正文语种 chi
中图分类人体细胞学;
关键词
造血干细胞; 基因表达; 病毒; 淋巴细胞;

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1. Correlation of a GVHD with CD4+CD25+ regulatory T cells of the donates and receipients in allogeneic hematopoietic stem cell transplantation [C] . Zhai Zhimin ,Sun Zimin ,Li Qing . 2005年华东六省一市血液病学学术会议暨浙江省血液病学学术年会 . 2005

In vivo anti-tumor activity of murine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene

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