Aim To investigate the effects of curcumol on the contractile activity of isolated duodenal smooth muscle in rats and explore its underlying mechanisms. Methods The isolated duodenum specimens of rats were made. The effects of different concentrations of curcumol on the contraction of isolated duodenal smooth muscle were observed using BL-420F biological and functional experimental system with constant tem-perature perfusion method. A certain concentration of curcumol was combined with atropine,isoproterenol, norepinephrine,calcium-free Krebs solution,verapam-il respectively to observe its effect on smooth muscle contraction. Results Curcumol could stimulate duo-denal smooth muscle in vitro,and increase its contrac-tile amplitude and tension significantly. It could be an-tagonized partly by atropine,isoprenaline,norepineph-rine,verapamil. Conclusion Curcumol can promote the contraction of isolated duodenal smooth muscle in rats,which may be achieved by stimulating M recep-tor,inhibiting α and β receptors,and promoting the extracellular calcium influx.%目的 观察莪术醇(curcumol)对大鼠离体十二指肠平滑肌收缩活动的影响,并探讨其可能的作用机制.方法 制作大鼠离体十二指肠标本,用恒温灌流的方法,使用BL-420F生物机能实验系统观察不同浓度莪术醇对大鼠离体十二指肠平滑肌收缩的影响.选取一定浓度莪术醇分别与阿托品、异丙肾上腺素、去甲肾上腺素、无钙Krebs液、维拉帕米共同孵育,观察其对平滑肌收缩的作用.结果 莪术醇可兴奋离体十二指肠平滑肌,使其收缩振幅和张力明显增大,该作用可被阿托品、异丙肾上腺素、去甲肾上腺素、维拉帕米部分拮抗.结论 莪术醇具有明显促进大鼠离体十二指肠平滑肌收缩的作用,其可能是通过兴奋M受体、抑制 α和 β受体、促进外钙内流实现.
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