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首页> 中文期刊> 《中国药理学通报》 >卡泊三醇诱导小鼠特应性皮炎模型的最适条件探索

卡泊三醇诱导小鼠特应性皮炎模型的最适条件探索

         
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摘要

Aim To investigate the method of establishing the atopic dermatitis mice model induced by calcipotriol ( MC903 ) . Methods Induction dose exploratory experiment: since d 0, 0.33,1,3 nmol MC903 was smeared on the right ear of BALB/c mice in each dose group respectively , for 7 consecutive days . The ear swelling degree of the mice was observed every day and the bilateral ears thicknesses were measured .The materials were drawn and analyzed in d 3 and d 7.Induction days exploratory experiment: 2 nmol MC903 was smeared on the right ear of BALB/c mice, for 14 consecutive days .The ear swelling degree of mice was observed every day and the bilateral ears thicknesses were measured .The histopathological examination of the right ear was conducted in 3 d, 7 d, 11 d and 15 d respectively .The tissue homogenate of the mice right ear was prepared .The ex-pressions of thymic stromal lymphopoietin (TSLP),IL-33, IL-4 and IFN-γin the homogenate , CD40 +,CD86 +,the DC surface markers and ILC2 contents in the peripheral lymph nodes were detected.Results ①1,3 nmol MC903 induced ear swelling in mice was significantly increased in d 3, the levels of TSLP and IL-4 were significantly increased .The level of IL-33 in 3 nmol dose group was increased significantly in d 7.② The right ear swelling of 2 nmol MC903 induced atopic dermatitis mice was significant , the ear thickness was increased gradually and reached the peak in d 14.The histopathological examination of the mice ear tissue showed in d 7, the right ear tissue of the mice was swelling and red , the capillary vessels were dilated and the infiltration of inflammatory cells was obvious .The ear in-flammatory symptoms maintained and gradually aggravated for 15 days.Compared with the normal mice , MC903 increased the TSLP level in the right ear tissue homogenate significantly in d 3 and then decreased gradually .The levels of IL-4 and IL-33 were increased significantly in d 7 and then decreased gradually .The levels of ILC2, CD40 +, CD86 + in the peripheral lymph nodes were increased in d 7 and d 15.Conclusion The atopic derma-titis mice model can be successfully established using 2 nmol MC903 induced mice for 7 days.Appropriate testing point of TSLP is d 3.Appropriate testing point of IL-33 and IL-4 is d 7.%目的探讨卡泊三醇（ MC903）致小鼠特应性皮炎模型的建立方法。方法诱导剂量探索实验：从d0起，于各剂量组BALB／c小鼠右耳分别涂布0.33、1、3 nmol MC903，连续诱导7d。每天观察小鼠耳肿胀程度并测量双耳耳厚，分别于d3、7取材分析。诱导时间探索实验：从 d 0起，于BALB／c小鼠右耳涂布2 nmol· ear－1 MC903，连续诱导至d 14。每天观察小鼠耳肿胀程度并测量双耳厚，分别于d 3、7、11、15获取小鼠右耳组织进行病理组织学检查。制备小鼠右耳耳组织匀浆，检测匀浆中胸腺基质淋巴细胞生成素（ TSLP）、IL-33、IL-4、IFN-γ表达水平以及外淋巴结中DC细胞表面标记分子CD40＋、CD86＋和ILC2细胞百分含量的变化。结果①每耳1、3 nmol MC903诱导的小鼠耳肿胀度从d 3开始显著增加， TSLP、IL-4水平明显增加，每耳3 nmol MC903剂量组IL-33水平于d 7明显增加。②每耳2 nmol MC903诱导的特应性皮炎小鼠从d 3起，小鼠右耳耳肿胀明显，耳厚逐渐增加，并于d 14达到峰值；小鼠耳组织病理组织学检查显示，从d7起，小鼠右耳耳组织红肿，毛细血管扩张，炎性细胞浸润明显，耳部炎性症状维持并逐渐加重至d 15；与正常小鼠相比， MC903使右耳组织匀浆中TSLP水平于d 3明显升高，随后逐渐下降，IL-4、IL-33水平于d 7明显升高，随后逐渐降低。外淋巴结中 DC 的表面标记分子CD40＋、CD86＋和ILC2的含量在d 7和d 15均有上升。结论每耳2 nmol MC903诱导小鼠7 d可成功建立小鼠特应性皮炎模型，TSLP较适检测点为d 3，IL-4、IL-33较适检测点为d7。

著录项

  • 来源
    《中国药理学通报》 |2016年第7期|1027-1032|共6页
  • 作者

    王璨; 王晓钰; 于曦; 陶羽; 王燕; 包凯帆; 季律; 洪敏;

  • 作者单位

    南京中医药大学药学院;

    科技部国家规范化中药药理实验室;

    江苏省中药药效与安全性评价重点实验室;

    江苏南京 210023;

    南京中医药大学药学院;

    科技部国家规范化中药药理实验室;

    江苏省中药药效与安全性评价重点实验室;

    江苏南京 210023;

    南京中医药大学药学院;

    科技部国家规范化中药药理实验室;

    江苏省中药药效与安全性评价重点实验室;

    江苏南京 210023;

    南京中医药大学药学院;

    科技部国家规范化中药药理实验室;

    江苏省中药药效与安全性评价重点实验室;

    江苏南京 210023;

    南京中医药大学药学院;

    科技部国家规范化中药药理实验室;

    江苏省中药药效与安全性评价重点实验室;

    江苏南京 210023;

    南京中医药大学药学院;

    科技部国家规范化中药药理实验室;

    江苏省中药药效与安全性评价重点实验室;

    江苏南京 210023;

    南京中医药大学药学院;

    科技部国家规范化中药药理实验室;

    江苏省中药药效与安全性评价重点实验室;

    江苏南京 210023;

    南京中医药大学药学院;

    科技部国家规范化中药药理实验室;

    江苏省中药药效与安全性评价重点实验室;

    江苏南京 210023;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 医用实验动物学;免疫细胞生物学;变态反应性及中毒性皮肤病;
  • 关键词

    卡泊三醇; 小鼠特应性皮炎; 胸腺基质淋巴细胞生成素; 白细胞介素-33; 白细胞介素-4; 树突状细胞; Ⅱ型固有淋巴细胞;

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