目的：研究海参皂苷对db/db肥胖小鼠血压的影响。方法将C57BL/KsJ(db/db)小鼠随机分为3组：模型组、海参皂苷低、高剂量组，并以 C57BL/KsJ小鼠作正常对照组。海参皂苷低剂量组小鼠摄入饲料中添加0．02%的海参皂苷，高剂量组饲料中添加0．04%的海参皂苷。连续饲喂6周，于第3周和第6周测定小鼠尾动脉血压，并分别测定肾脏血管紧张素转换酶(ACE)、血管紧张素转换酶2(ACE2)以及天冬氨酰蛋白酶( REN)基因的mRNA表达量。结果模型组小鼠血压高于正常组(P<0．01)，海参皂苷低剂量组血压低于模型组，但差异无显著性，海参皂苷高剂量组低于模型组(P <0．05)，且与正常组相近；海参皂苷的摄食对ACE、REN mRNA表达量无影响，海参皂苷高剂量组 ACE2 mRNA表达水平升高( P<0．05)。结论海参皂苷可改善肥胖进展过程中的血压升高，其机制可能与肾素-血管紧张素系统( RAS)的调节作用有关。%Aim To investigate the effects of saponin of sea cucumber ( SSC ) on the blood pressure in obese mice. Methods C57BL/KsJ(db/db) mice were ran-domized into 3 groups ( 8 mice each ): model group, low-dose SSC group and high-dose SSC group. Normal C57BL/KsJ mice were used as control. The low and high SSC groups were fed on basal diets incorporated with 0. 02% and 0. 04% SSC. Different treatments were administered for 6 weeks and arterial pressure was measured in the third and sixth weeks. The abundance of renal ACE, ACE2 and REN mRNA was detected by real time PCR . Results Compared with control group, the blood pressure of model group mice was ob-viously raised ( P<0. 01 ) . Low-dose SSC group mice showed lower blood pressure than model group without statistically significant differences, and the blood pres-sure of high-dose SSC group mice was similar to that of control group and significantly lower than model group. ( P<0. 05 ) There were no remarkable differences a-bout ACE and REN mRNA among the groups, howev-er, ACE2 mRNA level was significantly increased in high-dose SSC group. Conclusion SSC plays a vital role in decreasing blood pressure, which probably re-lates to the regulating function of renin-angiotensin sys-tem( RAS) .
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