Aim To study the mechanism of DXM and NAC inhibiting the expression of IL-8 and ICAM-1 in A549 cells. Methods The expression of IL-8 and ICAM-1 was detected by ELISA and flow cytometry re-spectively; the expression of GR,HDAC,AP-1,NF-κB was detected by Western blot, while the activity of HDAC was detected by spectrophotometry. ResultsThe increasing expression of IL-8 and ICAM-1 induced by TNF-α could be inhibited by DXM and NAC in A549 cells. DXM could inhibit the transcribed activa-tion of AP-1,NF-κB, and the expression of HDAC and its activity induced by TNF-α and LPS; NAC only in-hibited the transcribed activation of NF-κB, while it had no affection on the transcribed activation of AP-1 and the expression of HDAC and its activity. Conclu-sions DXM and NAC both have the anti-inflammatory effect. DXM plays the role of anti-inflammation through increasing the expression and activation of HDAC, in-hibiting the transcribed activation of AP-1 and NF-κB, while NAC has no effect on the expression and activa-tion of HDAC, which shows that NAC does not exert anti-inflammatory effect through acetylation signal.%目的：探讨 DXM 及 N-乙酰半胱氨酸( NAC )抑制A549细胞IL-8、ICAM-1表达的机制。方法 ELISA及流式细胞术分别检测IL-8及ICAM-1表达；蛋白印迹法检测GR、HDAC、AP-1、NF-κB表达，分光光度法检测HDAC活性。结果 DXM、NAC均能抑制 TNF-α诱导的 IL-8、ICAM-1表达增高。 DXM能抑制TNF-α、LPS诱导的AP-1及NF-κB转录活化、HDAC 表达及活性降低；NAC 只抑制 NF-κB 转录活化，对AP-1转录活化、HDAC表达及活性降低无影响。结论 DXM、NAC均具有抗炎作用。 DXM通过增加HDAC蛋白表达及活性，抑制AP-1、NF-κB转录活化发挥抗炎作用，而NAC对HDAC蛋白表达及活性无影响，提示NAC可能不是通过乙酰化信号机制发挥抗炎作用。
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