AIM To study the effects of vitamin E succinate (VES) on the cell growth and the DNA synthesis of human gastric carcinoma cell (SGC-7901). METHODS The growth curve was determined with counting viable cell numbers. The colony formations were counted with Giemsa dye staining. The cell cycle was analyzed using flow cytometry (FCM) and the DNA synthesis was observed with the 3H-TdR incorporation method. RESULTS VES could inhibit the growth and colony formation of SGC-7901 cells. Growth curve display：after SGC-7901 cells were treated with 5 mg*L-1、10 mg*L-1 and 20 mg*L-1 VES for seven days, the inhibition rate are 41.2%、98.3% and 100%, respectively. The colony formation of SGC-7901 cell at 24 h was inhibited 6.7%、50.4%、87.2%, and at 48 h was 24.7%、73.4%、100%, respectively. FCM analysis revealed that VES could decrease the percentage of cells in G2-M phase after treated 48 h in a dose-dependent manner, while increase the percentage of cells in S pheise. The assays of 3H-TdR incorporation into DNA showed obvious inhibition dose-dependently after exposure to VES for 48 h. CONCLUSION VES could inhibit gastric carcinoma cell growth by arresting DNA synthesis in vitro.%目的研究维生素E琥珀酸酯（VES）对人胃癌细胞 (SGC-7901) 生长以及DNA合成的影响。方法以体外培养的人胃癌细胞(SGC-7901)作为研究对象，用活细胞计数方法绘制生长曲线。Giemsa染色方法计数细胞形成集落数。用流式细胞仪检测VES对SGC-7901细胞周期的影响。并用3H-TdR参入方法研究VES对SGC-7901细胞DNA合成的影响。结果 VES可抑制SGC-7901细胞的生长和集落形成： 5 mg*L-1、10 mg*L-1和20 mg*L-1 VES处理SGC-7901细胞7 d后，细胞生长抑制率分别为41.2%、98.3%和100%；同样剂量的VES处理SGC-7901细胞24和48 h后，集落形成抑制率分别为6.7%、50.4%、87.2%和24.7%、73.4%、100%。细胞周期分析显示VES作用细胞48 h后，G2-M期细胞比例降低，并呈一定的剂量效应关系；同时S期细胞比例升高。在培养24和48 h后VES对SGC-7901细胞3H-TdR参入均有明显抑制作用，且呈剂量效应关系。结论 VES在体外可通过抑制细胞DNA合成来抑制人胃癌细胞生长。
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