目的 利用体外细胞共培养模型,探讨肥大细胞对前列腺癌细胞增殖及侵袭、转移的作用.方法 选取肥大细胞瘤P815细胞系及前列腺癌LNCaP细胞,使用24孔Transwell小室构建2种细胞体外共培养模型.四甲基偶氮唑蓝(MTT)比色试验检测前列腺癌LNCaP细胞的增殖,反转录定量聚合酶链反应(qRT-PCR)方法和蛋白印迹法检测前列腺癌LNCaP细胞E-cad、N-cad、Vimentin的表达.结果 前列腺癌LNCaP细胞与不同浓度肥大细胞共同培养12 h后吸光度(A)值变化与对照组差异无统计学意义(P>0.05),共同培养24 h及48 h后A值显著高于对照组,差异有统计学意义(P0.05), but was significantly higher at 24h and 48h (P<0.05). The experimental group showed signifi-cantly down-regulated E-cad expression and up-regulated N-cad and Vimentin expression, with significant difference compared with the control group (P<0.05). Conclusion Mast cells can promote the proliferation and the epithelial-mesenchymal transition of prostate cancer cells, and may also promote the invasion and metastasis of prostate cancer cells.
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