目的 探讨左旋多巴(L-DOPA)对脂多糖(LPS)诱导小胶质细胞产生致炎因子的影响及黄芪多糖(ASP)的保护作用.方法 不同浓度LPS(1、5、10 ng/ml)和(或)L-DOPA(50、100、500 μmol/L)及ASP (20 μg/ml)+LPS(10 ng/ml)+L-DOPA(50 μmol/L)处理小胶质细胞培养液,于0、4、24、48 h动态观察一氧化氮(NO)的含量、肿瘤坏死因子-α(TNF-α)的水平及黄芪多糖对上述指标的影响.结果 LPS处理小胶质细胞培养液,当LPS浓度5 ng/ml 48 h、10 ng/ml 24 h、L-DOPA浓度为100 μmol/L 72 h、500 μmol/L 24 h以后、LPS+L-DOPA组各个时间点NO的含量均高于对照组(P＜0.05),LPS+L-DOPA组显著高于单独用LPS或L-DOPA处理组(P＜0.05).LPS 5 ng/ml 24 h、10 ng/ml 4 h、L-DOPA500 μmol/L 72 h、LPS+L-DOPA组各时间点TNF-α的含量均高于对照组(P＜0.05),LPS+L-DOPA组显著高于单独用LPS或L-DOPA处理组(P＜0.05),黄芪多糖处理组明显降低NO的含量和TNF-α的水平(P＜0.05).结论 LPS致炎作用有时间剂量依赖性,小剂量L-DOPA无致炎作用,但L-DOPA增强LPS的致炎作用,黄芪多糖通过抑制NO、TNF-α的释放发挥抗炎保护作用.%Objective To explore effect of L-DOPA on inflammatory factors releasing induced by LPS and the protective effect of Asragulus Polysacharin (ASP). Methods Cultured microgliawere treated with LPS(1,5 and 10 ng/ ml) or/and L-DOPA(50,100,500 μmol/L), ASP 20 μg/ml and LPS(10 ng/ml)+L-DOPA(50 μmol/L). Changes of NO level and TNF-α level were dynamic observed at 0, 4, 24, 48 h, as well as the protective effect of ASP. Results When treated with LPS of 5 ng/ml after 48 h, of 10 ng/ml after 24 h, L-DOPA of 100 αmol/L after 72 h or 500 αmol/ L after 24 h, NO level was significantly higher than that of control(P<0.05); Treated with LPS+L-DOPA of 50 μmol/L, NO level was significantly higher than that of control; NO level of LPS+L-DOPA was significantly higher than group treated with LPS or L-DOPA alone (P<0.05). Of LPS 5 ng/ml 24 h, 10 ng/ml 4 h, L-DOPA 500 μl 72 h, LPS+L-DOPA group, TNF-α level was significantly higher than that of control (P<0.05 )in any hour; TNF-a level were significantly higher than group treated with LPS or L-DOPA (P<0.05). APS could significantly decrease NO and TNF-α level(P<0.05). Conclusions Inflammation effect induced by LPS was time- and dose-dependent. Low-dose L-DOPA may not be pro-inflammatory but can enhance the inflammatory effect induced by LPS. APS could inhibit NO and TNF-α releasing.
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