首页> 中文期刊> 《中国科学》 >Oligo(p-phenylenevinylene)-rhodium complex as intracellular catalyst for enhancing biosynthesis of polyhydroxybutyrate biomaterials

Oligo(p-phenylenevinylene)-rhodium complex as intracellular catalyst for enhancing biosynthesis of polyhydroxybutyrate biomaterials

         

摘要

Microbial synthesis utilizes sustainable resources to produce valuable chemicals,as a potential alternative to petroleum-based chemical industry.Although metabolic engineering is an efficient method to enhance the biosynthesis efficacy of microorganisms,it requires complicated biological procedures.Herein,we report a facile intracellular catalysis system for augmenting the production of bio-based material in microorganism.Covalent linking of oligo(p-phenylenevinylene)(OPV)and cyclopentadienyl rhodium(Ⅲ)bipyridine offers intracellular metal catalyst(OPV-Rh).The OPV-Rh complex displayed certain resistance to toxic biomolecules,which guaranteed its catalytic activity in complicated biological systems.With uptake by Gramnegative bacterium Ralstonia eutropha H16(R.eutropha H16),the OPV-Rh complex promotes the transformation of intracellular NADP+to NADPH,which further enhances the biosynthesis of polyhydroxybutyrate(PHB)by this microorganism.This work demonstrates that synthetic metal catalyst can be employed for regulating microbial biosynthesis intracellularly.

著录项

  • 来源
    《中国科学》 |2021年第1期|P.143-150|共8页
  • 作者单位

    Bejing National Laboratory for Molecular Sciences Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences Beijing 100190 ChinaCollege of Chemistry University of Chinese Academy of Sciences.Beijing 100049 China;

    Bejing National Laboratory for Molecular Sciences Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences Beijing 100190 ChinaCollege of Chemistry University of Chinese Academy of Sciences.Beijing 100049 China;

    Bejing National Laboratory for Molecular Sciences Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China;

    Bejing National Laboratory for Molecular Sciences Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China;

    Bejing National Laboratory for Molecular Sciences Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences Beijing 100190 ChinaCollege of Chemistry University of Chinese Academy of Sciences.Beijing 100049 China;

    Bejing National Laboratory for Molecular Sciences Key Laboratory of Organic Solids Institute of Chemistry Chinese Academy of Sciences Beijing 100190 ChinaCollege of Chemistry University of Chinese Academy of Sciences.Beijing 100049 China;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 物理化学(理论化学)、化学物理学;
  • 关键词

    microbial synthesis; rhodium complex; NADPH; PHB;

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