Objective To investigate the effect of epidermal growth factor (EGF) on proliferation and migration ability of amelanotic melanocytes (AMMC) in human hair follicle outer root sheath.Methods AMMC were purified to the third generation by the variant enzyme method.Then AMMC was identified by the immunohistochemical method;the changes of cellular proliferation and migration ability after intervention by different concentrations of EGF were detected by using CCK8 and Tianswell in vitro chamber migration test.Results The third generation AMMC immaro histochemical staining showed NKI/beteb staining positive and HMB-45 staining negative.The OD values of CCK8 test and the Transwell in vitro chamber migration assay results showed that its promoting proliferation and migration effects were increased with the EGF concentration increase,but when further increasing concentration,the effects had no obvious enhancement.Conclusion A certain concentration range of EGF in vitro can promote AM-MC proliferation and migration.%目的 观察表皮生长因子(EGF)对毛囊外毛根鞘(ORS)无色素性黑素细胞(AMMCs)增殖及迁徙能力的影响.方法 差胰酶法传代纯化AMMC至第3代;免疫组织化学染色法鉴定AMMC;CCK8检测法及Transwell体外小室迁移实验分别检测不同浓度EGF干预后细胞增殖、迁移能力变化.结果 第3代AMMC免疫组织化学染色显示NKI/beteb染色阳性,HMB-45染色阴性;CCK8法OD值及Transwell体外小室迁移实验结果显示,随EGF浓度升高,其促迁移及增殖的作用增加,但进一步增加浓度效果无明显增强.结论 体外一定浓度范围的EGF可促进AMMC增殖和迁移.
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