Objective To investigate the feasibility of P16 immunohistochemistry combined with routine pathology in judging the residual lesion of high grade cervical intraepithelial neoplasia.Methods Patients with cervical conization for high grade cervical intrapithelial neoplasia in this hospital from January 2014 to May 2016 were chose and divided into P16 immunohistochemical detection combined with pathological diagnosis group and pathological evaluation group according to patient's motivation.Patients with residual margins were treated in accordance with the clinical guidelines and TCT was followed up for 6 months after no margin.Then sensitivity and accuracy of two group were analyzed by gold standard of follow-up results.Results 104 patients in P16 immunohistochemical detection combined with pathological diagnosis group were negative in TCT test after 6 month of following up after surgery.However,at the time of 6 months follow-up after surgery,7 patients of 112 patients have been diagnosed with positive by TCT in pathological evaluation group.The Sensitivity and accuracy in P16 immunohistochemical detection combined with pathological diagnosis group were 100％ which were higher than pathological evaluation group.Conclusion P16 immunohistochemical detection combined with conventional pathology can accurately diagnose the cervical cutting edge of conization.%目的 探讨P16免疫组织化学检测联合常规病理评判高级别宫颈上皮内瘤变(CIN)锥形切除后切缘病灶残留的可行性.方法 选择2014年1月至2016年5月到该院就诊的CIN行宫颈锤形切除的患者,根据患者的意愿分为P16免疫组织化学检测结合常规病理评价组和常规病理评价组评判切缘是否存在病灶残留.切缘残留的患者按照临床指南给予相应处理,在切缘无残留后6个月进行宫颈液基诱层细胞学(TCT)检测随访.以随访结果为金标准,分析两组灵敏度和准确率.结果 P16免疫组织化学检测结合常规病理评价组104例患者术后6个月TCT检查均为阴性,单纯常规病理评价组112例患者术后6个月TCT检查发现7例患者阳性.P16免疫组织化学检测结合常规病理评价组的灵敏度和准确率均达到100％,显著高于常规病理评价组.结论 P16免疫组织化学检测结合常规病理能够准确地判断高级别CIN锥形切除患者的切缘情况.
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