Objective To investigate the effect of two different odontoblast inducer on the proliferation and apoptosis of rat adiposed-derived stem cells.Methods Adiposed-derived stem cells were collected by enzyme digestion from inguinal fat pads of 4 days post natal mice.Immunocytochemistry was performed to identify the cells.MTT and flow cytometry were tested the prolifera-tion and apotosis of adiposed-derived stem cells by co-cultured with tooth germ cell conditioned medium(TGC-CM)or dentin non-collagenous protein medium (DNCPM).Results Cells displayed a fibroblast-like appearance and positively expressed CD44 and CD105 when cufured to the secend yeneration.After 3 day the cells polarity changed by co-cultured.Count of cells were no obvious change by TGC-CM co-cultured,while that ruduced significantly by DNCPM co-cultured.It confirmed that the proliferation rate of ADSCs in TGC-CM group and control group is higher than DNCPM group(P <0.05),while the apotosis of that in DNCPM group is higher TGC-CM group(P <0.05).Compared with control group,the results had no significant difference(P >0.05).Conclusion TGC-CM may have more advantage as inducer in rat adiposed-derived stem cells differentiate into dentin like cells than DNCPM.%目的：探索2种不同的成牙本质诱导液———牙胚条件培养液（TGC-CM）和牙本质非胶原蛋白（DNCPM）对大鼠脂肪干细胞增殖和凋亡的影响。方法（1）取出生4 d 的 SD 乳鼠4只，分别取腹股沟脂肪组织，用酶消化法进行脂肪干细胞分离和培养，免疫细胞化学法对细胞进行鉴定。（2）2种诱导液作用细胞后，倒置相差显微镜下观察细胞形态变化，MTT 法检测细胞增殖情况，Annexin／PI 双染后流式细胞仪检测细胞的凋亡情况。结果（1）细胞培养至第2代时表型分子 CD44和 CD105表达呈阳性。（2）经 TGC-CM 及 DNCPM 培养3 d 后形态上发生极性改变。培养6 d 后，TGC-CM 培养的细胞数量未见明显改变，而DNCPM 培养的细胞密度明显降低。（3）MTT 结果显示 DNCPM 组 OD 值低于对照组及 TGC-CM 组（P ＜0．05），流式细胞凋亡检测仪检测 DNCPM 组细胞凋亡率高于 TGC-CM 组和对照组（P ＜0．05）。结论作为大鼠脂肪干细胞向成牙本质样细胞方向分化的诱导液，TGC-CM 可能更具优势。
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