Objective To observe sorafenib induced apoptosis on human hepatoma cell line HepG2 in vitro and to explore ex pression and significance of Mcl 1 and bFGF 2. Methods HepG2 cells were treated with gradient of different concentrations of sor afenib with the untreated cells used for control. The cell killing effect of the treatment was determined by CCK 8 kit,and the rate of the cell apoptosis was detected by flow cytometry. Elisa was used for detecting the bFGF 2. Western blot was performed to deter mine the expressions of Mcl 1 in the cells. Results Compared with the control group,sorafenib inhibited the proliferation and in duced apoptosis of HepG2 cells,and efficiency of high concentration group was higher than other groups(P＜0. 05). The bFGF 2 of higher group was lower than other groups(P＜0. 05). Conclusion Sorafenib can inhibit the proliferation,and induce the apoptosis of human hepatoma cell line HepG2. Over expression of Mcl 1 and bFGF 2 may play an important role in the genesis and progres sion of hepatoma cells.%目的 观察索拉非尼体外诱导人肝癌细胞HepG2的凋亡效应,以及对髓样细胞白血病-1蛋白质(Mcl-1)及人碱性成纤维细胞生长因子-2(bFGF-2)的表达的影响.方法 采用不同浓度梯度的索拉非尼作用于人肝癌细胞株HepG2,CCK-8试剂盒测定细胞杀伤效应,流式细胞术检测肝癌细胞凋亡率,采用Western blot测定Mcl-1表达,ELISA法检测细胞培养液中bFGF-2的水平.结果 与对照组相比,索拉非尼能诱导人肝癌细胞株HepG2凋亡,呈一定的浓度时间效应(P＜0.05),Mcl-1及bFGF-2表达均减少.结论 索拉非尼可抑制人肝癌细胞株HepG2增殖,诱导其凋亡,Mcl-1及bFGF-2可能在肝癌细胞的增殖分化中起着重要的作用.
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