Objective The purpose of present study is to investigate the influence of thalidomide on gene expression and transcription of bone sialoprotein (BSP) in ROS17/2.8 cells.Methods ROS17/2.8 cells were randomly divided into control group and thalidomide group (10μg/mL thalidomide).Real-time PCR was conducted to detect the BSP mRNA levels.Transient transfection assay was used to measure the BSP transcription activities.Results The BSP mRNA level was significantly decreased by thalidomide(P＜0.05).Thalidomide significantly depressed the transcription activity of BSP promoter pLUC3,4 (P＜0.05).Conclusion 10 μg/mL thalidomide suppressed BSP gene expression and transcription in ROS17/2.8 cells.%目的 研究沙利度胺作用成骨细胞(ROS17/2.8)后对骨唾液酸蛋白(BSP)基因表达和转录活性的影响.方法 将ROS17/2.8细胞随机分为两组:空白对照组、沙利度胺组(10μg/mL沙利度胺),各组持续作用12 h后,采用实时荧光定量PCR检测BSP信使RNA(mRNA)的表达,用瞬时转染法检测各组的荧光素酶活性,分析BSP基因启动子的转录活性.结果 沙利度胺组的BSP mRNA表达量与空白对照组的表达量取比值,沙利度胺使比值明显降低,差异有统计学意义(P＜0.05)；沙利度胺组BSP启动子(pLUC3、4)的荧光素酶活性值即转录活性值与空白对照组的转录活性值取比值,沙利度胺使比值明显降低,差异有统计学意义(P＜0.05).结论 沙利度胺抑制了ROS17/2.8细胞中BSP基因启动子的基因表达和转录.
展开▼
