在棉花现蕾期筛选到一个高抗黄萎病的内生菌菌株,根据16S rDNA序列同源性,将其命名为Paenibacillus xylanilyticus YUPP-1(解木聚糖类芽孢杆菌YUPP-1).当把该菌标记后,每株棉花施用该菌株达到1×106 cfu时,该菌能顺利定殖于棉花体内.室内盆栽抗病实验表明:当生防菌灌根处理的菌量为每株1×106,1×107,1×108 cfu,其在结铃期的发病株率仅为8％,5％和2％,而此时对照的发病株率高达96％.2008-2009年的小区防治结果表明:当生防菌灌根处理的菌量为每株1×108 cfu时,2008年处理区的棉花黄萎病发病株率和病指为14.8％和8.5,对照分别为53.3％和26.5,2009年处理区的棉花黄萎病发病株率和病指为9.4％和6.5,对照为37.8％和18.8.这些结果表明,解木聚糖类芽孢杆菌YUPP-1对防治棉花黄萎病具有巨大的应用潜力.%To control cotton wilt caused by Verticillium dahliae Kleb, we collected endophytic antagonistic microorganisms during different growth stages and assayed the combined effects. One strain with strong activity against V. Dahliae was isolated from cotton seedlings. This strain was preliminarily identified as Paenibacillus xylanilyticus YUPP-1 by 16S rDNA sequence analysis. This strain was able to successfully colonize cotton roots when it was at concentrations of more than l×l06 cfii per cotton seedling. A pot experiment of the effect of Paenibacillus xylanilyticus YUPP-1 on cotton Verticillium wilt showed the following: in comparison with a control with an infection rate of 96%, only 8%, 5%, and 2% infection rate was observed for the treatments with strain YUPP-1, 1 × 106, 1 × 107, and 1×105 cfii per cotton seedling, respectively. Field tests indicated that control of this disease could be achieved when strain YUPP-1 reached 1 x 10* cfu per cotton seedling. We have thus demonstrated that P. Xylanilyticus YUPP-1 is an effective endophytic biocontrol agent for Verticillium wilt.
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