AIM: A near-infrared diffuse reflectance analysis (NIRDRA) method was developed for rapid non-destructive quantitative determination of clarithromycin in capsule. METHOD:All spectra were measured with a near-infrared spectrometer equipped with a PbS detector, an external integrating sphere and a rotating sample cup. Each sample was put into the sample cup and was scanned from 12*#000 cm -1 to 4*#000 cm -1 , and each sample spectrum was obtained as an automatic mean of 64 scans. No spectrum pre-processing method was used, and a spectral region, 7*#502 ～5*#446 cm -1 , was selected to develop a mathematical model by partial least square method. RESULT: The optimal rank and mean square error determined by cross validation method was 7 and 0.00306, respectively. The average recovery and RSD of clarithromycin was 100% and 1.33%, respectively. CONCLUSION: Results showed that the NIRDRA method was rapid, simple, nondestructive and sensitive. It can be applied to predict the content of clarithromycin in capsule.
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