Objective To develop a PCR method to distinguish Citrobacter freundii which agglutinate with O157 serum from Escherichia coil O157. Methods All the strains isolated were subjects for biochemical identification, test for agglutination with O157 serum and test for wzx gene by PCR. Results The wzx gene was detected in 25 strains of Citrobacterfreundii which showed agglutination reaction with O157 serum, while no wzx gene was detected in 8 strains of Citrobacterfreundii which showed no agglutination reaction with O157 serum. No wzx PCR product was detected in 20 strains of O157: H7. Conclusion The wzx-basod PCR is a sensitive and rapid method to distinguish Citrobacterfreundii strains which agglutinate with OI57 serum from Escherichia coli O157 strains.%目的 利用基于O抗原翻转酶wzx基因的PCR方法,鉴别与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌和O157大肠埃希菌.方法 用微生物鉴定仪对细菌进行生化鉴定,与P157血清凝集,同时扩增wzx基因.结果 25株与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌及1株不与大肠埃希菌O157血清凝集的弗氏枸橼酸杆菌wzx基因扩增为阳性,8株与O157血清不凝集的弗氏枸橼酸杆菌wzx基因扩增为阴性,20株O157:H7大肠埃希菌wzx基因扩增为阴性.结论 对于可与O157血清发生强凝集的弗氏枸橼酸杆菌,基于O抗原翻转酶wzx基因的PCR方法是一种有效的快速鉴别方法.
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