目的:建立同时测定苯巴比妥、苯妥英钠和卡马西平血药浓度的反相高效液相色谱法.方法:采用Diamonsil Sum C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈:水(V:V=33:67),检测波长为230 nm,流速为1.0 ml/min,柱温为30℃,进样量为20μl.结果:苯巴比妥、苯妥英钠、卡马西平的血药浓度在1.0~80.0μg/ml范围内呈良好的线性关系(r>0.99),日内和日间精密度RSD均0.99) .The intra-and inter-day precision ( RSD) was no more than 15%.The average recovery of PPB , PHT and CBZ were respectively 92.2%, 89.1% and 89.4%.CONCLUSIONS: The method was simple , sensitive and accurate .It was suitable for clinica1 monitoring of PPB , PHT and CBZ in serum .
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