目的：提升知百安神口服液的质量标准。方法：采用薄层色谱法分别对知母和百合进行定性鉴别，采用高效液相色谱法对知母新芒果苷、芒果苷进行定量检测。色谱柱为Agilent Eclipse XDB-C18（4.6 mm ×250 mm，5μm），流动相为乙腈-0.2％甲酸水溶液，采用梯度洗脱，流速为1 ml／min，检测波长为246 nm，柱温为30℃，进样量为20μl。结果：薄层鉴别色谱中斑点特征明显、专属性好。新芒果苷、芒果苷的质量浓度线性范围分别为10.5～210.0、10.4～208.0μg／ml（r2均≥0.9999），精密度、重复性、稳定性试验的RSD均＜2.00％。平均加样回收率分别为103.49％（ RSD＝0.86％）、98.81％（ RSD＝1.26％）（ n＝6）。结论：该方法简便可靠，可用于提升知百安神口服液的质量控制。%OBJECTIVE:To improve the quality standard of Zhibaianshen oral lquid .METHODS: Anemarrhenae rhizoma and lilii bulbus were identified by TLC method , and the concentration of neomangiferin and mangiferin were determined by HPLC method.Separation was carried out on a Agilent Eclipse XDB-C18 (4.6 mm ×250 mm, 5μm) column with the mobile phase of acetonitrile and 0.2%formic acid in gradient elution at a flow rate of 1 ml/min.The detection wavelength was set at 246 nm, the column temperature was 30 ℃, and the injection volume was 20 μl.RESULTS:The characteristics of spots in TLC were quite clear with strong specificity .As for neomangiferin and mangiferin , the linear range of the calibration curve was 10.5-210.0 and 10.4-208.0 μg/ml, respectively (r2≥0.999 9), and the results of precision , accuracy and stability were satisfactory with RSD less than 2.00%.The average recovery rate turned out to be 103.49%( RSD=0.86%) and 98.81%( RSD=1.26%) ( n=6 ) .CONCLUSIONS: The established method is simple and reliable , which can be used for the quality control of Zhibaianshen oral liquid .
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