OBJECTIVE:To establish a method for the content determination of Vitamin B1 , Vitamin B2 in the Compound hepar extractum tablets.METHODS: HPLC method was adopted, the chromatographic column was SHIMADZU shim-packVP-ODS(250 mm ×5 mm, 4.6μm) , the mobile phase was methanol-organic solution( V∶V=9∶91) , the determine wavelength was 266 nm, with flow rate of 1.0 ml/min.RESULTS: Vitamin B1 had a good linearity in the range of 7.65-153.15 μg/ml and Vitamin B2 was 2.54-50.85 μg/ml.The regression equation of Vitamin B1 was Y=94.16 X+10.34, r=1.000, and Vitamin B2 was Y=34.22 X+29.61, r=1.000.The average recovery rates were accord with the requirements.CONCLUSIONS:The method is simple, fast and accurate, and can be used as the quality control method of Compound hepar extractum tablets.%目的：建立复方肝浸膏片中维生素B1、维生素B2的含量测定方法。方法：采用反相高效液相色谱法测定，色谱柱为SHIMADZU shim-packVP-ODS（250 mm ×5 mm，4.6μm）；流动相为甲醇-有机溶液（ V ∶V＝9∶91）；检测波长为266 nm；流速为1.0 ml／min。结果：维生素B1质量浓度在7.65～153.15μg／ml范围内、维生素B2质量浓度在2.54～50.85μg／ml范围内的线性关系良好，回归方程维生素B1为Y＝94.16 X＋10.34，r＝1.000；维生素B2为Y＝34.22 X＋29.61，r＝1.000；加样回收率符合要求。结论：该方法操作简便、快捷、结果准确，可作为复方肝浸膏片的质量控制方法。
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