To explore the anti-senile effect of Ganoderma lucidum polysaccharides (GLP)Probiotic Yogurt,D-galactose induced aging mice were fed with Probiotic Yogurt containing different proportion of GLP.Viscera index,SOD,CAT,GSH-Px activities and MDA contents in serum and tissue samples were measured.The results showed that SOD,CAT and GSH-Px activity were significantly increased and MDA content decreased in serum and tissue of male mice fed with GLP Probiotic Yogurt,while the high dose group [1 200 mg/(kg · d)] had the optimal results.Compared with the aging model group,in the group of male mice fed with 1 200 mg/(kg · d) of the GLP Probiotic Yogurt,SOD activities in serum,liver and brain were respectively increased by 20.34％,8.39％,48.95％,GSH-Px activities in serum and liver were respectively increased by 38.45％,39.67％,MDA contents in serum,liver and brain tissue were respectively reduced by 38.37％,43.97％,38.39％,CAT activities in the brain,liver and kidney were respectively increased by 60.02％,55.29％,37.73％.Compared with the yogurt group,SOD activities of the high dose group were respectively increased by 8.39％,1.58％,25.13％.Their GSH-Px activities wererespectively increased by 38.45％ and 39.67％.MDA contents were reduced by 43.97％,30.96％,and 18.91％,respectively.CAT activities were increased by 55.29％,32.07％ and 26.64％.In conclusion,the synergy of ganoderma lucidum polysaccharides (GLP) and probiotic yogurt could have a potential anti-aging function.%对D-半乳糖致衰老小鼠分别灌胃添加不同含量灵芝多糖的益生菌酸奶,测定各组处理小鼠脏器指数,血清及不同组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)活性指标,研究添加灵芝多糖的益生菌酸奶对D-半乳糖致衰老小鼠抗衰老作用的效果.结果表明:添加灵芝多糖的益生菌酸奶可显著提高衰老模型小鼠血清及不同组织中SOD、CAT、GSH-PX活性、降低MDA含量(P＜0.05),且以高剂量组[1 200 mg/(kg·d)]效果最优.与衰老模型组相比,高剂量组[1 200 mg/(kg· d)]小鼠血清、肝脏、脑组织中SOD活力依次提高20.34％、8.39％、48.95％;小鼠血清、肝脏中GSH-PX活力依次提高38.45％、39.67％;小鼠血清、肝脏、脑组织中MDA含量分别降低38.37％、43.97％、38.39％;小鼠脑组织、肝脏、肾脏中CAT活力依次提高60.02％、55.29％、37.73％.与酸奶组相比,高剂量组小鼠SOD活力依次提高18.36％、1.58％、25.13％;GSH-PX活力依次提高38.45％、30.26％;MDA含量分别降低10.47％、30.96％、18.91％;CAT活力依次提高41.31％、32.07％、26.64％.结论:灵芝多糖添加入植物乳杆菌益生发酵酸奶可发挥灵芝多糖和益生菌酸奶在抗衰老功能方面的协同作用发挥更强抗衰老效果.
展开▼
