腺苷是合成阿糖腺苷、腺苷酸(AMP)、三磷酸腺苷(ATP)的主要原料,是一种重要的医药原料.目前,微生物发酵生产腺苷越来越受到重视,会产生大量的发酵液样品需要检测.基于腺苷脱氨酶(ADA)建立了一种快速、高通量检测发酵液腺苷的方法.实验结果表明,响应面方法优化检测试剂浓度为碳酸钠-碳酸氢钠34.20mmol/L-27.40 mmol/L,亚硝基铁氰化钠3.35 g/L,次氯酸钠的有效氯为0.172 5％,百里香酚6.64 g/L.该方法检测灵敏,检测限为0.025～6 mmol/L,能够满足发酵检测和高通量筛选的需要.%Adenosine is the main raw material for the production of vidarabine,adenosine monophosphate (AMP) and adenosinetriphosphate (ATP).Adenosine is also an important pharmaceutical raw materials.At present,microbial fermentation production of adenosoine will produce large amounts of fermentation samples to be detected A rapid high -throughput method for detecting adenosine in fermentation broth based on adenosine deaminase (ADA)was estabished.Using response surface model analysis to determine the optimum concentration of four substances,the results showed sodium carbonate-sodium bicarbonate 34.20 mmol/L-27.40 mmol/L,sodium nitropmssid 3.35 g/L,the effective chlorine of sodium hypochlorite 0.172 5％,thymol 6.64 g/L.The limit of detection was between 0.025 mmo1/L and 6 mmo1/L,this method is sensitive and can meet the needs of the fermentation detection and high-throughput screening.
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