Frozen abalone visceral were used as materials. The lyzed. The optimized conditions of ultrasound-assisted glycoprotein general components of abalone visceral were anaextraction was selected by orthogonal analysis on the basis of single factor experiments. The optimized conditions of ultrasound-assisted extraction were as follows ： NaCl concentration was 5 % ,the ratio of material to solvent was 1 ： 9, ultrasonic power was 300 W and ultrasonic time was 40 min. The extraction rate of glycoprotein in the extraction was 69.7mg/g by this method, the extraction ratio increased by 28. 5% compared to the tradition method. Furthermore, the glycoproteins were precipitated by （NH4）2SO4 and isolated by DEAE-Cellulose and SephacrylS-300 HR. Results showed that a homogeneous glycoprotein （ AGP- Ⅱ ） was obtained. The molecular weight of AGP-Ⅱ was estimated to be 73.7 ku by SDS-PAGE. AGP- Ⅱ contained 12.4% total sugar, 76.6% protein.%以冻藏鲍鱼脏器为试验原料，分析了鲍鱼脏器的基本成分组成，研究了鲍鱼脏器糖蛋白的分离纯化及纯化物基本组成。通过单因素试验、正交试验确定了最适提取条件为：5％NaCl溶液、料液质量比1：9、超声功率为300W、超声处理时间为40min，在此条件下，糖蛋白提取率为69．7mg／g（干重），比传统方法提高了28．5％。糖蛋白粗品经硫酸铵沉淀，DEAE—Cellulous52柱层析，SephacrylS-300HR柱层析后，经SDS—PAGE电泳鉴定得到分子质量为73．7ku的组成均一的糖蛋白AGP-Ⅱ，AGP—Ⅱ含有总糖12．4％，总蛋白76．6％。
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