The article analyzed the glucooligosaccharides synthesized with microwave-assistance by HPLC and IR and studied its surface hydrophobicity (S_0). We also studied the scavenging activity of glucooligosaccharides with different degrees of polymerization to·OH radical, DPPH radical and ABTS~+·radical. The results showed that the scavenging activity of glucooligosaccharides to ·OH radical and DPPH radical increased with the degree of polymerization , concentration and surface hydrophobicity increase. The scavenging activity of HGOS was the best and its lC_(50)was about 66 mg/mL and 44 mg/mL. The scavenging activity to Water-soluble ABTS~+·radical reduced with the degree of polymerization, concentration and increased surface hydrophobicity. The IC_(50)50 of LGOS and MGOS were 74. 7 mg/mL and 78 mg/mL. The best scavenging activity of HGOS was 48. 3% .%对微波合成葡寡糖(GOS)进行了高效液相色谱(HPLC)、红外光谱(IR)、表面疏水性(S_0)分析,研究了3种不同聚合度葡寡糖(LGOS、MGOS、HGOS)对·OH、DPPH、ABTS~+·3种自由基的清除作用.结果表明:葡寡糖对·OH自由基、DPPH自由基清除作用随着聚合度、浓度及疏水性的增加而增强,HGOS的清除能力最强,IC_(50)分别为66 mg/mL和44 mg/mL;对水溶性自由基ABTS~+·清除能力随着葡寡糖聚合度、浓度及疏水性的增加呈降低趋势,LGOS、MGOS对ABTS~+·自由基的IC_(50)分别为74.7 mg/mL和78 mg/mL,HGOS的最高清除率达到48.3%.
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