以6个品种3种色系葡萄的果梗、果皮及部分品种的葡萄籽为材料,采用有机溶剂法提取不同品种葡萄的果梗、果皮和种籽中的白藜芦醇,利用HPLC测定提取液中白藜芦醇的含量,并分析了白藜芦醇对DPPH自由基和羟自由基的清除能力.结果表明,葡萄不同品种和组织间白藜芦醇含量差异显著,整体表现为果梗>果皮>种籽,果皮紫黑色品种>红色>黄绿色;金田0608和夏黑果梗白藜芦醇含量较高(22.4~22.6 μg/g),阳光玫瑰和白罗莎里奥果梗藜芦醇含量很低(1.2~1.8 μg/g).除葡萄籽白藜芦醇含量与DPPH自由基清除率呈显著正相关外,其它组织白藜芦醇含量与自由基清除率无显著相关性.金田0608和夏黑果梗、果皮白藜芦醇提取液清除DPPH自由基和羟自由基的能力较强.%The stems, skin and some varieties of grape seeds from six varieties classified as three kinds of color grape were used to extracted the resveratrol by organic solvent and then detected its content by HPLC.Moreover, the effect of resveratrol on DPPH radical and hydroxyl radical scavenging ability were also studied.The results showed that the resveratrol content in different grape varieties and tissue distributed differently, and the volume decreased in fruit stem, skin, and pip orderly.Furthermore, it was also found that the resveratrol content in the varieties of purple-black was the highest in the fruit skin, but it was the lowest in the varieties of yellow-green.The resveratrol content in stem of 'Jintian 0608' and 'Summer Black' was higher (22.4~22.6 μg/g), but it was very low (1.2~1.8 μg/g), in those of 'Sunshine Rose' and 'White Rosario' stems.It was confirmed that the resveratrol content in grape pip was significantly positively related to DPPH free radical clearance, however no significant correlation was found between the content of other tissues and this clearance.Finally, the resveratrol extracts from the fruit stem and skin of 'Jintian 0608' and 'Summer Black' were found showing stronger ability in cleaning both DPPH and hydroxyl radicals than those of the other tissues.
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