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松籽壳多糖超声辅助溶剂法提取及抗氧化性研究

         

摘要

Study on ultrasonic-solvent assisted extraction technology of soluble polysaccharuie from pine nut shell. Through single factor test and orthogonal experiment L9 ( 34 ) optimize extraction process. The removal efficiency of pine nut shell polysaccharide on hydroxyl radicals (OH · ) and superoxide anion (O2- · ) detected was investigated through removal experiment. The result demonstrate that the ultrasonic-assisted extraction optimum technological conditions as follows: ultrasonic time 30. 0 min, extraction temperature 60 ℃, solid-liquid ratio 1 : 50(m : V) , and ultrasonic extraction times 1; the pine nut shell polysaccharides have a strong scavenging capacity effect on hydroxyl radicals(OH · ) ,but a general scavenging capacity effect on superoxide anion (O2- · ).%用超声波辅助溶剂法提取松籽壳中可溶性多糖,通过单因素和正交试验L9(34)优化提取工艺.并通过清除试验研究松籽壳多糖清除羟基自由基(·OH)和超氧阴离子自由基(O2-·)的效果.结果表明:超声波辅助提取松籽壳多糖的最佳工艺条件为超声时间30 min,提取温度60℃,料液比1∶50(m∶V),超声提取次数1次,该条件下多糖含量为44.26%;松籽壳多糖具有较强的清除(·OH)能力,对(O2-·)的清除作用一般.

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