试验采用超声波辅助提取灵芝发酵粉中三萜类化合物,运用分光光度法测定其得率.通过单因素和Box-Benhnken设计研究乙醇浓度、浸泡时间、料液比、超声时间、超声功率、超声温度对灵芝三萜类化合物得率的影响.研究结果表明,灵芝三萜化合物提取的最佳工艺参数为:料液比为1:42(g/mL),95 %乙醇溶液中浸泡100 min后,超声功率为210 W下43℃超声提取17 min.在最优工艺条件下进行3次验证试验,测得三萜化合物平均得率为0.6128 mg/g.研究结果为灵芝发酵类产品的在线监测提供理论依据.%Triterpene compounds in Ganoderma lucidum powder were extracted by ultrasonic wave and the yield was determined by spectrophotometry. The effects of ethanol concentration, soaking time, solid-liquid ratio, ultrasonic time, ultrasonic power and ultrasonic temperature on the yield of Ganoderma lucidum triterpenoids were studied respectively.Based on the single-factor experiments,and ultrasonic power as significant factors,according to the design principle of Box-benhnken,the response surface of the experiment optimization design.The optimal process parameters were as follows:the ratio of solid to liquid was 1:42(g/mL),immersed in 95 % ethanol for 100 min,the temperature of ultrasound was 43℃,and the ultrasonic power was 210 W for 17min.validation experiments were carried out at these optimum conditions,the average yield of triterpenoids measured was 0.612 8 mg/g.
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