以黑皮鸡枞为试验材料,以黑皮鸡枞多糖提取得率为考察指标,首先对超声波法提取多糖的料液比、提取时间、提取温度及超声波功率进行了单因素试验,在此基础上,采用四因素三水平正交试验进一步对黑皮鸡枞多糖提取工艺进行了优化.结果表明:黑皮鸡枞多糖的最佳提取工艺为料液比为1:40(g/mL),提取时间40 min,温度为85℃,超声波功率160 W,在此条件下,黑皮鸡枞多糖提取得率为7.9%.%Ter-mitomycesHeim was used as experimental material, the extraction rate of polysac charide from Ter-mitomycesHeim was as examining index, single factor test of material liquid ratio, exaction time, exac-tion temperature and ultrasonic power were studied. On the basis of this, four factors and three levels orthogo-nal test were used to optimize the extraction process of polysaccharide from Ter-mitomycesHeim. The re sult showed:material liquid ratio 1:40 (g/mL), 40 min, 85℃and ultrasonic power 160 W were the optimal extrac-tion process.Under these conditions, the extraction rate of polysaccharide from Ter-mitomycesHeim was 7.9%.
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