采用高效液相色谱法(high performance liquid chromatography,HPLC)、液相色谱-质谱法(liquid chromatography-mass spectrometry,LC-MS)鉴定红枣低聚糖成分,建立一种测定多种糖分的HPLC分析方法,测定不同品种红枣低聚糖含量.样品经70％(体积分数)乙醇提取,Ca柱HPLC分离分析,采用阳离子检测模式的LC-MS进行定性分析,峰面积外标法进行定量分析.通过HPLC保留值和LC-MS法鉴定出红枣中含有蔗果三糖和蔗果四糖.建立的HPLC分析方法可使红枣的5种主要糖分实现很好的分离,在0.30 mg/mL～10.0 mg/mL之内线性关系良好,r=0.999 6 (n=5),RSD为0.53％～0.83％,加标回收率为97％～108％,对32种红枣的低聚糖进行含量测定,结果显示5种糖分总含量为223 mg/g F.W～395 mg/g F.W,其中金丝新1号含量最高.两种低聚糖含量范围为3.1 mg/g F.W～14.8 mg/g F.W,其中阜平大枣含量最高.蔗果三糖和蔗果四糖是红枣中代表性低聚糖,在成熟期果实中其含量在1％(鲜重)数量级.%High performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) for determination of the oligosaccharides in jujube were used.A method based on HPLC was established for simultaneous determination of various saccharides in jujube,to determine the content of oligosaccharides in different kinds of jujube.the samples were extracted by 70 ％ ethanol,the separation was achieved on a HPLC with Ca-column,the qualitative analysis was achieved on a LC-MS with cationic mode,the external calibration curves were used in quantification.Kestose and nistose trihydrate were identified by the retention values of HPLC and LC-MS.5 kinds of main saccharides were satisfactorily separated by the established HPLC method,the linear range was 0.30 mg/mL-10.0 mg/mL (r =0.999 6),the relative standard deviations (RSD) of repeated measurement were between 0.53 ％-0.83 ％,standard addition recovery rate was between 97 ％ 108 ％.By the method,we analyzed the content of jujube oligosaccharides from 32 different varieties,it turned out that the total content of 5 saccharides was between 223 mg/g F.W-395 mg/g F.W,the highest content of the jin si xin NO.1,the total content of 2 oligosaccharides was between 3.1 mg/g F.W-14.8 mg/g F.W,the highest content of the fuping jujube.Kestose and nistose trihydrate were the representative oligosaccharides in jujube,the content reached up to 1％ in the mature period.
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