以猪血为原料,以DPPH自由基清除率为指标,采用酶法制备抗氧化肽并用响应面分析法(RSM)优化酶解条件.试验结果表明,冰浴+超声波粉碎的血红细胞破碎率高达97.1％,试验条件下水解猪血红蛋白制备抗氧肽的最适用酶为碱性蛋白酶,在温度58℃、pH7.7、加酶量6.5％条件下的酶解产物对DPPH自由基的实际清除率达96.94％,与模型预测值基本相符.%Taking pig blood as raw material and scavenging rate of DPPH free radical as index,antioxidants peptide was prepared with enzymatic hydrolysis method,and the optimal enzymatic hydrolysis conditions were achieved by response surface methodology.Results showed that broken rate of red blood cells could reach 97.1％ by treatment of ultrasonication in ice bath,alkaline protease was selected as the better enzyme for preparation of antioxidant peptides from hydrolyzed pig haemoglobin.Under the optimal enzymatic hydrolysis conditions of temperature 58 ℃,pH 7.7,enzyme concentration 6.5 ％,the actual scavenging rate of DPPH free radical reached 96.94 ％,which was consistent with the predicted results.
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