以海藻酸钠为载体,戊二醛为交联剂,对L-阿拉伯糖异构酶进行固定化.研究海藻酸钠浓度、氯化钙浓度、戊二醛浓度以及硬化时间等因素对固定化效果的影响.结果表明最佳固定化条件为:海藻酸钠浓度3.5%,氯化钙浓度3.0%,戊二醛浓度0.2%,硬化时间7 h,该条件下制备的固定化酶与游离酶相比,最适宜反应温度和pH值基本没有变化,并且具有较好的操作稳定性和贮存稳定性.%L-alabinose isomerase (L-AI)was immobilized using sodium alginate as the carrier and flutaralde-hyde as the crosslinker. The effects of the concentration of sodium alginate, calcium chloride and flutaralde-hyde, the solidifying time were studied. The results showed that the optimal immobilization condition were as followed: sodium alginate 3.5 %, calcium chloride 3.0 %, flutaraldehyde 0.2 % and the solidifying time 7 h. Under the optimum condition, compared with the free L-AI, the optimum pH value and temperature of immo-bilized L-AI were not changed. The immobilized L-AI had good operational stability and storage stability.
展开▼
