研究了D-苯基乳酸高产菌株在5L发酵罐中全细胞转化苯丙酮酸钠合成D-苯基乳酸的条件,分别考察了溶氧量、表面活性剂、底物补加方式等对D-苯基乳酸产量的影响.结果表明:最适溶氧为40%,控制pH值为7.0分批补加底物得到D-苯基乳酸的产量为18.76 g/L,较未控制pH值发酵提高7.22%;添加1%吐温80时,D-苯基乳酸产量达到了18.59 g/L,比未加表面活性剂发酵提高了6.98%.在此基础上,将底物匀速流加得到D-苯基乳酸的产量提高到19.43 g/L.%The conditions of the synthesis of D-phenyllactic acid by the whole cell transformation of phenylpyruvic acid in 5 L fermentor were studied in this paper. Dissolved oxygen, surfactant, substrate adding method on the yield of D-phenyllactic acid were studied. The results showed:the optimum dissolved oxygen was 40%, the yield of D-phenyllactic acid was 18.76 g/L when batch addition of the substrate and a constant pH of 7.0, compared with natural pH fermentation increased by 7.22%. The yield of D-phenyllactic acid was up to 18.59 g/L when 1%Twain was added, compared with that without surfactant addition increased by 6.98%. On the basis of this, the yield of D-phenyllactic acid was increased to 19.43 g/L under the uniform substrate flow.
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