探讨以芦丁作为对照品，建立三氯化铝（AlCl3）比色法测定火棘总黄酮含量的最优条件。系统考察了对照品、光照、反应时间及温度、显色剂AlCl3浓度及用量、体系pH、缓冲液用量等因素对总黄酮与AlCl3显色反应的影响。结果表明，AlCl3比色法测定火棘总黄酮含量的最优条件为：1 mL适宜浓度的火棘提取液，依次加入2 mL pH5.4的醋酸-醋酸钠缓冲液，1 mL质量浓度2%AlCl3液，70%乙醇定容至10 mL并混匀，不避光条件下30℃反应12 min，285 nm测定吸光度。该测定方法具有良好的重复性、稳定性、重现性，回收率。%Rutin was taken as reference substance and the optimal condition of aluminum chloride colorimetric assay affecting the determination of total flavonoids from Pyracantha fortuneana extract was established. Several parameters, such as the standard substance, light, reaction time and temperature, concentration and the amount of chromogenic agent, pH and buffer dosage that affecting the color reaction of total flavonoids were investigated systematically. The results indicated the appropriate reaction conditions were as the following:2 mL 2 %(m/m) Aluminum chloride was added to 1 mL pyracantha extract with specific concentration, after being blended the reaction was carried out at 30℃for 12 min, then absorbance was measured at 285 nm. As a result, Statistical analysis showed the method had good repeatability, stability, reproducibility and high recovery rate.
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