At the liquid culture conditions, the fermentation medium of GC 1325, the high yielding of L-arginine strain mutagenized form brevibacterium flavum optimized using single factor test. Different concentrations of carbon sources, nitrogen sources, inorganic salts, and thiamine effect on the production of L-arginine were studied. The initial concentration of carbon sources , nitrogen sources and additional way were confirmed. The experimental results show that the optimal fermentation medium for GC 1325:glucose 100 g/L, (NH4)2SO4 25 g/L,KH2PO4 1.2 g/L,MgSO4·7H2O 0.6 g/L, biotin 100μg/L;Glucose (50 g/L) fed once at 24 h and 25 % aqueous ammonia fed continuously to maintain the (NH4)2SO4 concentration was 25 g/L in the fermentation process. At this optimization of culture conditions, the maximum yield of L-arginine was 37.8 g/L, which was increased by 58.8%.%在液体培养条件下，采用单因素试验法，考察了发酵培养基中不同浓度的碳源、氮源、无机盐和生物素对实验室筛选获得的黄色短杆菌L-Arg高产突变株GC 1325产酸的影响，确定了碳源和氮源的初始浓度和补加方式。实验结果表明，GC 1325发酵的最优培养基为：葡萄糖100 g/L，（NH4）2SO425 g/L、KH2PO41.2 g/L、MgSO4·7H2O 0.6 g/L、生物素100μg/L；发酵过程中24 h后一次流加50 g/L葡萄糖维持碳源；连续流加25%的氨水维持（NH4）2SO4浓度在25 g/L水平，在此优化的培养条件下， GC 1325获得的最大的L-Arg产量为37.8 g/L，比优化前提高了58.8%。
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