建立了同时测定奶粉香兰素、甲基香兰素和乙基香兰素的超高效液相色谱法。样品用水溶解后使用ProElut PXA固相萃取柱进行样品前处理净化，C18柱分离，超高效液相色谱仪进行色谱分析。结果表明，香兰素、甲基香兰素和乙基香兰素在0.5 mg/L~20 mg/L范围内线性关系良好；方法的检出限（S/N=3）分别为0.20、0.25、0.25 mg/L；添加水平在2.5 mg/L~10.0 mg/L时加标回收率为80.3%~102.7%，相对标准偏差为1.85%~4.11%（n=6）。该方法结果准确可靠，可实现奶粉中香兰素、甲基香兰素和乙基香兰素的快速同步分析。%An ultra performance liquid chromatography (UPLC) method was developed for the simultaneous determination of vanillin, methyl vanillin and ethyl vanillin in milk powder. Samples were dissolved with water, purified with solid phase extraction column, separated with C18 column, and determined with ultra performance liquid chromatography. The results showed that there were good linear relationships between peak areas and concentrations of vanillin, methyl vanillin and ethyl vanillin in the range of 0.5 mg/L-20 mg/L, with limits of detection of 0.20 mg/L,0.25 mg/L and 0.25 mg/L, respectively. The recoveries at spiked levels of 2.5 mg/L-10.0 mg/L were in the range of 80.3%-102.7%, with the relative standard deviations (RSDs, n=6) of 1.85%-4.11%. The method was sensitive and reliable, and could achieve the fast simultaneous analysis of vanillin, methyl vanillin and ethyl vanillin in milk powder.
展开▼
