得到脂肪酶固定化的最佳条件及固定脂肪酶对生物柴油的转化率.利用单因素试验和正交试验确定脂肪酶固定化的最佳条件,通过气相色谱-质谱联用仪分析合成生物柴油的主要成分.结果:脂肪酶固定化的最佳条件:缓冲液pH 7.5、载体/酶液比为25 mg/mL、固定化温度应为40℃,其固定化酶活力为68.25 U/g,利用固定脂肪酶催化合成生物柴油的转化率高达75%.结果表明:固定化酶比游离酶具有更高的催化效率,对高温和pH具有更高的耐受性.固定化脂肪酶催化效率高、操作稳定性较强,具有较好的工业化生产潜力.%It was to get the best conditions of lipase immobilization and the conversion rate of biodiesel production. The optimum conditions of lipase immobilization were obtained by single factor experiment and orthogonal test. The main components of the biodiesel were analyzed by the gas chromatograph-mass spectrometer. The optimum conditions of lipase immobilization were obtained that was the pH of buffer was 7.5 , the ration of vector : enzyme solution was 25 mg : 1 mL, the immobilized temperature was 40 ℃. The immobilized enzyme activity was 68.25 U/g. With immobilized lipase the conversion rate of biodiesel production was up to 75%. The result showed that the immobilized lipase had higher catalytic efficiency and its tolerance was more improved to high-temperature and pH than the free enzyme. The immobilized lipase had a potential for industrial production because of its high catalytic efficiency and strong operational stability.
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