建立高效液相色谱法同时测定骏枣中芦丁、槲皮素和异鼠李素的测定方法.采用Kromasil C18色谱柱；甲醇-0.2%磷酸(60∶40,体积比)为流动相；流速1 mL/min；检测波长370 nm.结果表明：芦丁回归方程y=726083x+1938(r=0.9998),在0.0383滋g~0.612滋g范围内线性关系良好,平均加样回收率99.34%,RSD=1.42%；槲皮素回归方程y=8667980.8x-2869.6(r=0.9999),在0.0058滋g~0.092滋g范围内线性关系良好,平均加样回收率99.82%,RSD=2.27%；异鼠李素回归方程y=325300x+2611.4(r=0.9992),在0.0275滋g~0.440滋g范围内线性关系良好,平均加样回收率98.90%,RSD=2.44%.该法操作简便、准确,重复性好,可用于骏枣中芦丁、槲皮素和异鼠李素含量的测定.%The contents of rutin, quercetin and isorhamnetin in Zizyphus jujuba cv. Jun were determined by high performance liquid chromatography (HPLC)usingKromasilC18 (150mm×4.6mm,5μm)column,Methanol-0.2%phosphoric aicd (60∶40,v∶v)as mobile phase at a flow rate of 1.0 mL/min and detection wavelength of 370 nm. The results showed that the linear range of rutin concentration was from 0.038 3 μg to 0.612μg, the regression equation was y=726 083x+1 938 (r=0.999 8), the average recovery was 99.34%with the RSD of 1.42%. The linear range of quercetin concentration was from 0.005 8 μg to 0.092μg, the regression equation was y=8 667 980.8x-2 869.6 (r=0.999 9), the average recovery was 99.82%with the RSD of 2.27%. The linear range of isorhamnetin concentration wasfrom 0.0275μg to 0.440μg, the regression equation wasy=325300x+2611.4 (r=0.9992), the average recovery was98.90%with the RSD of 2.44%. The proposed method had highly repeatability and selectivity. It was simple,fast andcouldbeusedforthedeteminationofrutin, quercetinandisorhamnetinin Zizyphusjujuba cv.Jun.
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