建立碱性橙II的酶联免疫分析方法。采用戊二醛偶联法将半抗原碱性橙II与载体牛血清白蛋白（BSA）及卵清蛋白（OVA）制备免疫抗原碱性橙II-BSA和包被抗原碱性橙II-OVA。经紫外可见吸收光谱鉴定和大白兔免疫实验证实人工抗原成功合成，并制备抗碱性橙II的多克隆抗体，该抗体可检测快速定量食品中的碱性橙II，最低检测限为10 pg。该检测方法具有高灵敏度和特异性，可应用于食品中碱性橙II检测试剂盒的研制。% In order to determined the Chrysoidin II by enzyme-linked immunosorbent assay (ELISA) method, a complete antigen Chrysoidin II -BSA and a coating antigen Chrysoidin II -OVA were prepared by coupling Chrysoidin II with the carrier proteins of bovineserum albumin (BSA)and ovalbumin (OVA) using glutaraldehyde method, respectively. The synthesis of Chrysoidin II artificial antigens was confirmed by UV absorption spectra and animal experiments. A novel anti-Chrysoidin II polyclonal antibody was prepared , which was successfully applied to the determination of Chrysoidin II in food with a limit of detection of 10 pg. High sensitivity and specificity will contribute to the development of commercial ELISA kits for the detection of Chrysoidin II in foods.
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