通过对样品中待测组分的提取、净化，建立一种快速测定潮汕猪肉丸中盐酸克伦特罗和莱克多巴胺两种β-受体激动剂残留量的液质联用检测方法。样品用β-葡萄糖醛苷酸酶溶液酶解，20 mmol/L乙酸铵缓冲溶液（pH5.2）提取，固相萃取柱净化，通过质谱对样品进行确证，外标峰面积法定量。方法加标平均回收率盐酸克伦特罗为98.6%～110.1%，试验的RSD为0.26%～0.93%；莱克多巴胺为90.1%～101.3%，试验的RSD为0.22%～1.33%。该方法优化了盐酸克伦特罗和莱克多巴胺的仪器分析条件，操作方便，重现性好，回收率高，结果准确，能满足食品安全控制的检测需要。% A method was developed for fhe determination of clenbuterol and ractopamine residues in Chaoshan pork balls by SPE-LC-MS/MS. The sample was extracted and purified by solid phase extraction(SPE)cartridge, analyzed by LC-MS/MS.The sample was hydrolyzed by β-glucuronic acid enzyme,extracted by ammonium acetate buffer solution (pH5.2)and purified by solid phase extraction (SPE)cartridge.finally,confirmed by mass spectrometry and the quantity is set by external standard peak area.The rate of average recovery of clenbuterol was 98.6%-110.1%,and RSDs ranged from 0.26%to 0.93%;ractopamine was 90.1%-101.3%,and RSDs ranged from 0.22 % to 1.33 %.In this paper,optimized the condition of LC-MS/MS,the method has the advantages of simplicity,rapidity,precision,good reproducibility and high recovery,so that it could be used for the determination of clenbuterol and ractopamine residues ,and it can meet the need of measuring in food safety control.
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