To develop an immunoassay for detection of oxaprozin ,oxaprozin was conjugated to BSA and OVA to synthesize artificial antigens using active ester method. Polyclonal antibodies were obtained from immunized rabbits. The IC50 value and LOD of the optimal ELISA were 3.1 ng/mL and 0.5 ng/mL,respectively. All cross-reactivities against 8 associate drugs were less than 0.1%. The ELISA was in good agreement with LC-UV when detected oxaprozin in 1 positive and 29 negative health liquors from market.% 建立快速检测违法添加剂奥沙普嗪的免疫学方法。以活泼酯法将奥沙普嗪分别连接到BSA和OVA上制备人工抗原,免疫家兔制备多克隆抗体,经条件优化建立竞争抑制酶联免疫吸附检测方法。结果显示:检测体系IC50值为3.1 ng/mL,最低检测限为0.5 ng/mL,与8种相关药物交叉反应率都小于0.1%。用ELISA方法与HPLC检测30种市售保健酒,1例阳性和29例阴性结果全部吻合。
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