To prolong the shelf life of aquatic products, denaturing gradient gel electrophoresis (DGGE) fingerprinting was used to study the dynamic changes of microorganisms in flounder during storage at low temperature. DNA of bacteria extracted from the fish was amplified by touchdown PCR. We got various of specific conserved microbial nucleotide sequences. The amplified nucleotide sequences of microorganisms were analysed with DGGE. The DGGE profiles showed that there were abundant microbial communities on the flounder in the initial storage period. With the extension of storage time , only a few bacteria survived. The analysed DNA sequences showed that the specific spoilage organisms of Paralichthys olivaceus were Shewanella putrefaciens, Pseudomonas spp., Aeromonas spp. and Serratia marcescens.%为延长水产品货架期，利用变性梯度凝胶电泳（DGGE）方法，研究了牙鲆在低温贮藏条件下主要微生物的动态变化。提取鱼肉中微生物核酸，进行降落PCR扩增，得到多种微生物特异性保守核酸序列。利用变性梯度凝胶电泳分析扩增出的微生物核酸序列。DGGE图谱表明，新鲜牙鲆在贮藏初期微生物群落较为丰富，随着贮藏时间增长，只有几种优势菌存在。经DNA序列分析表明，牙鲆的特定腐败菌为腐败希瓦氏菌（Shewanella putrefaciens）、假单胞菌属（Pseudomonas spp.）、气单胞菌属（Aeromonas spp.）、粘质沙雷氏菌（Serratia marcescens）。
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