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首页> 外文期刊>Journal of environmental sciences >Dynamic changes of microbial community diversity in a photohydrogen producing reactor monitored by PCR-DGGE
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Dynamic changes of microbial community diversity in a photohydrogen producing reactor monitored by PCR-DGGE

机译：PCR-DGGE监测光生氢反应器中微生物群落多样性的动态变化

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A PCR-DGGE (denaturing gradient gel electrophoresis of polymerase chain reaction) protocol was used for monitoring the dynamic changes in the microbial population during photohydrogen production. Total DNA was extracted directly from the mixed bacterial community in the reactor and subjected to PCR with V3-16S rDNA and pufM gene primers, and the amplifications were then analyzed by DGGE. The DGGE patterns demonstrated the dynamics of community structure and the shift of microbial diversity, which corresponded to different running periods of the reactor. The optimal hydrogen producing community formed on day 10. Using DGGE analysis with the pufM gene fragments was superior to V3-16S rDNA region genes for detecting the dynamic variations of the photosynthetic bacteria population during hydrogen production. The comparative sequence analysis of excised DGGE bands showed the relationship between specific population structures and system performance. Rhodopseudomonas palustris was presumed as one of the dominant community members for hydrogen production in the reactor. The PCR-DGGE protocol was proven to be a good tool for monitoring the photohydrogen production in real time and offered the available information to improve the photohydrogen producing system.
机译：PCR-DGGE（聚合酶链反应的变性梯度凝胶电泳）方案用于监测光氢产生过程中微生物种群的动态变化。从反应器中的混合细菌群落中直接提取总DNA，并用V3-16S rDNA和pufM基因引物进行PCR，然后通过DGGE分析扩增。 DGGE模式展示了群落结构的动态变化和微生物多样性的变化，这对应于反应堆的不同运行时间。在第10天形成了最佳的产氢群落。使用DGGE分析pufM基因片段优于检测V3-16S rDNA区域基因，以检测产氢过程中光合细菌种群的动态变化。切除的DGGE带的比较序列分析显示了特定种群结构与系统性能之间的关系。据推测，大红假单胞菌是反应器中氢气生产的主要社区成员之一。 PCR-DGGE协议被证明是实时监测光氢产生的一个很好的工具，并提供了可用于改进光氢产生系统的可用信息。

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