We used the independently developed electrode modified by polypyrrole , to determine the content of vitamin E in vegetable oil. The uncertainty was calculated by analysing the affecting factors in the detection process, such as weighing, glass container, standard curve, electrodes, recovery, and so on. The results indicated that, the oil sample whose vitamin E test results was 258.18 mg/kg, was 7.36 mg/kg for the relative standard uncertainty and 5.7%for the relative expanded uncertainty , and the uncertainty was mainly caused by type A uncertainty and uncertainty of the glass container.%利用自主研制的聚吡咯修饰电极测定植物油中VE的含量，通过对检测过程中称量、玻璃量器、标准曲线、电极以及回收率等影响不确定度的因素进行分析，计算不确定度。结果表明，VE检测结果为258.18 mg/kg的油样，其相对标准不确定度为7.36 mg/kg，相对扩展不确定度为5.7%，不确定度主要由a类不确定度与玻璃量器的不确定度引起。
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