以玉米肽(corn peptide,CP)为参照研究玉米肽-锌(CP-Zn)螯合物的结构表征和体内抗氧化活性变化.采用氨基酸分析、紫外扫描、红外光谱研究结构变化;对小鼠灌胃不同剂量的CP、CP-Zn(低、中、高剂量组分别为50、150、250 mg/ (kg·d)),测其血清、肝脏中丙二醛(malondialdehyde,MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力,分析比较CP、CP-Zn体内抗氧化活性.结果表明:螯合前后氨基酸组成、紫外吸收光谱、红外吸收光谱存在明显变化,Zn2+与-COOH、-NH2、C=O进行了配位;CP高剂量、CP-Zn高、中剂量能显著降低小鼠血清、肝脏中MDA含量(P＜0.05或P＜0.01),提高SOD活力(P＜0.01)、GSH-Px活力(P＜0.05或P＜0.01),CP-Zn抗氧化活性优于CP.由此可见,CP-Zn属于螯合物,同时具有增强CP抗氧化活性的作用.%In this work,in comparison to corn peptide (CP),we investigated the change in structural characteristics and antioxidant activity in vivo of corn peptide-Zn (CP-Zn) complex.The structures of CP and CP-Zn were investigated through amino acid analysis,ultraviolet (UV) spectroscopy and infrared spectroscopy.CP and CP-Zn (50,150,250 mg/(kg.d)) at different doses were administered intragastrically to mice.Two days later,malondialdehyde (MDA) content,superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity in serum and liver were measured to evaluate antioxidant activity in vivo of CP and CP-Zn.The results showed a significant change in amino acid content,and UV and FT-IR spectra between CP and CP-Zn.Zn2+ was coordinated with the-COOH,-NH2 and C =O groups of corn peptide.Compared with the control group,high-dose CP and high-and middle-dose CP-Zn could reduce MDA level (P ＜ 0.05 or P ＜ 0.01),and improve SOD (P ＜ 0.01) and GSH-Px activity (P ＜ 0.05 or P ＜ 0.01) in serum and liver.The antioxidant activity of CP-Zn was better than that of CP.
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