以杜仲叶片cDNA为模板,采用反转录RCR及RACE技术分离出DXR基因cDNA全长.序列分析结果表明该基因序列全长l 814 bp,共编码478个氨基酸,推导的蛋白质分子量为51.71 kD,理论等电点5.79,命名为EuD-XR.推导的EuDXR蛋白质序列具有植物DXR酶的5个典型基序,并预测出26个潜在的功能位点.系统进化树分析表明EuDXR蛋白与玉米、水稻亲缘关系最近,其次为橡胶、拟南芥、烟草.%Homologous DXR gene cDNA was isolated from leaves of Eucommia ulmoides by method of reverse transcription polymerase chain reaction ( RT-PCR ) and rapid amplification of cDNA ends ( RACE ) technique, and named after EuDXR. The nucleotide sequence of EuDXR was 1 814 bp and contains an open reading frame (ORF) of 1 437 bp encoding 478 amino acids with a molecular weight of about 51. 71 kD,and the theoretical isoelectric point of which was 5.79, representative motifs of plant DXR and 26 potential functional sites were deduced in the a-mino acids sequence of EuDXR. The results of phylogenetic analysis suggested that the protein sequence of EuDXR was more similar to that of Zea mays and Oryza saliva than other species, and followed by Hevea brasiliensis, Arabi-dopsis thaliana and Nicotiana tabacum.
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