In this experiment gene -targeting GFP transgenic zebrafish 189 b was used to observe the whole development process of lateral line system and the responses to neomycin treatment.The results showed that the occurrence and distribu-tion of GFP-labeling cells were highly consistent with the spatiotemporal development of the neuromasts along the lateral line, especially in migration route and final residence.As the larvae grew , the diffused migratory GFP -cells focused as small and bright GFP-dots.Exposure of 24 h and 6 d of GFP-positive embryos in 150 μmol/L of amino sugar glucoside class antibiotic ( neomycin ) did not change the GFP -pattern and distribution.These results suggested that the GFP -labe-ling cells might be neuromast progenitors and resistant to neomycin.%利用转基因斑马鱼（Danio rerio）189b动态观察侧线系统形成及其对新霉素处理的敏感性。试验结果显示，转基因斑马鱼荧光细胞出现、迁移和定居与侧线神经丘的形成走向和发育时间基本吻合。随着发育时间的推移，荧光点的形态也发生了较大变化，由最初的比较模糊的聚集在一起的荧光团，逐渐变成颗颗分明的荧光点，大小也由大变小。将受精后24 h和6 d带有荧光的细胞暴露于150μmol/L的氨基糖甙类抗生素（新霉素）中，并不影响荧光细胞的数量和分布。结果说明荧光标记细胞对新霉素处理不敏感，不是毛细胞，可能是神经丘形成的先祖细胞。
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