通过同源克隆从木薯品种辐选01(RS01)中获得 SPS 基因保守序列,利用 RACE 扩增技术获得全长cDNA 序列并使用相关软件进行生物信息学分析,并采用实时荧光定量 PCR 分析木薯 SPS 基因在不同时期、不同组织中的相对表达量。结果表明：克隆获得的木薯 SPS 基因 cDNA 序列全长3857 bp,开放阅读框(ORF)长3228 bp,编码1076个氨基酸,命名为 MeSPS (GenBank 登录号 KX822780)；同源性分析表明,MeSPS 基因与麻风树、蓖麻和荔枝的 SPS 基因序列同源性较高,分别为89%、89%和87%；其氨基酸序列与其他植物SPS 氨基酸同源性在77%~92%；经 qRT-PCR 分析结果表明,MeSPS 在苗期的相对表达量较高；在同一生长时期,MeSPS 在叶片中相对表达量高于茎段和块根。%Sucrose phosphate synthase (SPS)gene from cassava RS01 was cloned to study its biological functions. The conserved fragment of the gene was first obtained by homologous cloning,and its full length cDNA by rapid amplification of cDNA ends (RACE).The characteristics,gene sequence and other biological information retrieved were analyzed.Expressions of the genes from various tissues of the plants at different times were compared by RT-qPCR.The results showed that the cDNA of SPS gene had a full length of 3,857 bp,and an ORF of 3,228 bp encoding a protein of 1,076 amino acids.It was subsequently named MeSPS with a GenBank number of KX822780. Its homologous alignments with the nucleotide and protein databases were highly homologs with those of J atropha carcas L.,Ricinus communis L.,and Litchi chinensis Sonn.at 89%,89%,and 87%,respectively.Its amino acid sequence was 77% - 92% in similarity with the SPS genes of other plants.The qRT-PCR analysis showed that MeSPS gene was highly expressed during seeding,and,was more expressed in the leaves than the stems or roots on a plant in a same growth period.
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