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首页> 美国卫生研究院文献>Journal of Insect Science >Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper Nilaparvata lugens Stål (Hemiptera: Delphacidae)
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Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper Nilaparvata lugens Stål (Hemiptera: Delphacidae)

机译：糖原磷酸化酶和糖原合成酶：基因克隆和表达分析揭示了它们在褐飞虱褐飞虱海藻糖代谢中的作用（半翅目：Delphacidae）

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RNA interference has been used to study insects’ gene function and regulation. Glycogen synthase (GS) and glycogen phosphorylase (GP) are two key enzymes in carbohydrates’ conversion in insects. Glycogen content and GP and GS gene expression in several tissues and developmental stages of the Brown planthopper Nilaparvata lugens Stål (Hemiptera: Delphacidae) were analyzed in the present study, using quantitative reverse-transcription polymerase chain reaction to determine their response to double-stranded trehalases (dsTREs), trehalose-6-phosphate synthases (dsTPSs), and validamycin injection. The highest expression of both genes was detected in the wing bud, followed by leg and head tissues, and different expression patterns were shown across the developmental stages analyzed. Glycogen content significantly decreased 48 and 72 h after dsTPSs injection and 48 h after dsTREs injection. GP expression increased 48 h after dsTREs and dsTPSs injection and significantly decreased 72 h after dsTPSs, dsTRE1-1, and dsTRE1-2 injection. GS expression significantly decreased 48 h after dsTPS2 and dsTRE2 injection and 72 h after dsTRE1-1 and dsTRE1-2 injection. GP and GS expression and glycogen content significantly decreased 48 h after validamycin injection. The GP activity significantly decreased 48 h after validamycin injection, while GS activities of dsTPS1 and dsTRE2 injection groups were significantly higher than that of double-stranded GFP (dsGFP) 48 h after injection, respectively. Thus, glycogen is synthesized, released, and degraded across several insect tissues according to the need to maintain stable trehalose levels.
机译：RNA干扰已被用于研究昆虫的基因功能和调控。糖原合酶（GS）和糖原磷酸化酶（GP）是昆虫体内碳水化合物转化的两个关键酶。本研究分析了褐飞虱Nilaparvata lugensStål（Hemiptera：Delphacidae）的几个组织和发育阶段的糖原含量以及GP和GS基因表达，使用定量逆转录聚合酶链反应来确定它们对双链海藻糖酶的反应。 （dsTREs），6-磷酸海藻糖合酶（dsTPSs）和有效霉素注射液。在翼芽中检测到两种基因的最高表达，其次是腿部和头部组织，并且在所分析的发育阶段显示出不同的表达模式。注射dsTPSs后48和72 h和注射dsTREs后48 h，糖原含量显着降低。注射dsTREs和dsTPSs后，GP表达增加48 h，而注射dsTPSs，dsTRE1-1和dsTRE1-2后，GP表达显着下降72 h。注射dsTPS2和dsTRE2后48 h和注射dsTRE1-1和dsTRE1-2后72 h GS表达显着下降。注射有效霉素后48 h，GP和GS的表达和糖原含量显着下降。注射有效霉素后48 h，GP活性显着下降，而注射后dsTPS1和dsTRE2组的GS活性显着高于注射后48 h的双链GFP（dsGFP）。因此，根据维持稳定的海藻糖水平的需要，糖原在几种昆虫组织中合成，释放和降解。

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