为探索一定浓度外源 Ca2＋在缓解枇杷冻害中所起到的积极作用，以盆栽枇杷苗为试材，先采用不同浓度 CaCl2溶液喷施叶片，并用焦锑酸钙沉淀法检验处理效果，后采用人工降温的方法进行低温处理，并对-3℃1.5 h 处理条件下的枇杷叶组织抗寒生理的相关指标进行测定。结果表明：0.05～50 mmol ・ L -1浓度的 CaCl2溶液，均能不同程度地降低枇杷苗 MDA 含量和提高 SOD 、 POD 、 CAT 及 APX 活性。其中，以10 mmol ・ L -1 CaCl2溶液处理的 SOD 活性最高，高于对照26.70％，且达到显著水平；以25 mmol ・ L -1 CaCl2溶液处理的 APX活性最高，高于对照6.39％，但未达到显著水平。结论：在低温来临之前果园喷水防寒时，喷施适宜浓度的CaCl2溶液有利于提高枇杷苗的抗寒性，从而可以在一定程度缓解低温伤害。%CaCl2 solution of different concentrattion were sprayed on the leaves of potted Eriobotrya japonica seedlings ,the effects were verified by the calcium pyroantimonate precipitation method . Then ,the seedlings were artificially treated at - 3 ℃ for 1.5 h ,and the cold resistance-relating physiological indices of the leaf tissues were measured . The results showed that CaCl2 solutions of 0.05 - 50 mmol ・ L - 1 could all managed to decrease the MDA contents and to increase the SOD ,POD ,CAT and APX activities . The SOD activity of the control group was significantly elevated by 26.70% by using 10 mmol ・ L - 1 CaCl2 solution . Although the APX activity of the control was maximally increased by 6.392% via 25 mmol ・ L - 1 CaCl2 solution , the upgrowth was still not significant . Thus ,spraying appropriate CaCl2 solution besides water in orchards prior to cold-air outbreaks was conducive to augmenting the cold-resistance indices of Eriobotrya japonica seedlings ,thereby minimizing the low-temperature damages .
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