This paper was aimed to investigate effects of different vernalization time on the growth of broad bean. With "Dapengyicun" bean as material, protective enzymes, MDA content and chlorophyll content of horsebean seedlings were tested at 0, 7, 14, 21, 28 days treated by vernalization. The results showed that as the increasing of vernalization time, activities of superoxide dismutase ( SOD ), peroxidase ( POD ) and catalase ( CAT ) all increased firstly and then decreased. SOD and CAT activities peaked at 14 days, while the POD activity was at 21 days; The Malondialdehyde ( MDA ) content rose slowly at the early stage, then increased substantially at 28 days. No significant difference was detected on chlorophyll content as vernalization time extending. The result showed that vernalization time for bean seedling was the best when controlled around 14 days, not more than 21 days.%为研究不同春化时间对蚕豆幼苗生长的影响,以“大朋一寸”蚕豆品种为材料,分别对蚕豆种子进行0、7、14、21、28 d的低温春化处理,待播种长成幼苗后分别取幼叶测量植物体内的保护酶活性、MDA含量、叶绿素的含量变化.结果表明:随着春化时间增长,蚕豆幼苗中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性整体呈现先上升后下降的趋势,其中SOD和CAT的活性在春化处理14d时最高,POD的活性在春化处理21d时最高；丙二醛(MDA)含量的整体趋势则是先缓慢上升,到春化处理28 d时大幅度上升；叶绿素的含量随着春化时间的延长,没有差异不显著.结果表明蚕豆种子春化的时间最好在14 d内,不宜超过21d.
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